Selected recent publications

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Question-driven research: ion channel signaling complexes

The Formation of Kv2.1 Macro-Clusters is Required for Sex-Specific Differences in L-type Cav1.2 Clustering and Function in Arterial Myocytes. 
Matsumoto, C. T., O’Dwyer, S. C., Manning. D. C., Hernandez-Hernandez, G., Rhana, P., Fong, Z., Sato, D., Clancy, C. E., Vierra, N. C., Trimmer, J. S., and Santana, L. F.  (2023).
Commun. Biol. 6:1165. PMID: 37963972.

Neuronal ER-Plasma Membrane Junctions Couple Excitation to Ca2+-Activated PKA Signaling.
Vierra, N. C., Ribeiro-Silva, L., Kirmiz, M., van der List, D., Bhandari, P., Mack, O. A., Carroll, J., Le Monnier, E., Aicher, S. A., Shigemoto, R., and Trimmer, J. S.  (2023).
Nat. Commun. 14:5231. PMID: 37633939.

NPC1-dependent Alterations in Kv2.1–Cav1 Nanodomains Drive Neurodegeneration.
Casas, M., Murray, K. D., Hino, K., Vierra, N. C., Simo, S., Trimmer, J. S., Dixon, R. E., and Dickson, E. J.  (2023).
Nat. Commun. 14:4553. PMID: 37507375.

Endoplasmic Reticulum-Plasma Membrane Junctions as Sites of Depolarization-Induced Ca2+ Signaling in Excitable Cells.
Dixon, R. E., and Trimmer, J. S. (2023).
Ann. Rev. Physiol.
85:217-243. PMID: 36202100.

Ion Channel Partnerships: Odd and Not-So-Odd Couples Controlling Neuronal Ion Channel Function.
Vierra, N. C., and Trimmer, J. S. (2022).
Int. J. Mol. Sci.
23:1953. PMID: 35216068.

Regulation of Neuronal Excitation-Transcription Coupling by Kv2.1-induced Clustering of Somatic L-type Ca2+ Channels at ER-PM Junctions.
Vierra, N. C., O’Dwyer, S. C., Matsumoto, C., Santana, L. F., and Trimmer, J. S. (2021).
Proc. Nat. Acad. Sci. USA  
118:e2110094118. PMID: 34750263.

Loss of the K+ Channel Kv2.1 Greatly Reduces Outward Dark Current and Causes Ionic Dysregulation and Degeneration in Rod Photoreceptors.
Fortenbach, C., Allina, G. P., Shores, C. M., Karlen, S. J., Miller, E. B., Bishop, H. I., Trimmer, J. S., Burns, M. E., and Pugh, E. N.,  Jr.  (2021).
J. Gen. Physiol. 153:e202012687. PMID: 33502442.

Epilepsy and Neurobehavioral Abnormalities in Mice with a Dominant-Negative KCNB1 Pathogenic Variant.
Hawkins, N. A., Misra, S. N., Jurado, M., Kang, S. K., Vierra, N. C., Nguyen, K., Wren, L., George Jr., A. L., Trimmer, J. S., and Kearney, J. A.  (2021).
Neurobiol. Dis.
147:105141. PMID: 33132203.

KCTD5, a Novel TRPM4-regulatory Protein Required for Cell Migration as a New Predictor for Breast Cancer Prognosis.
Rivas, R., Díaz, N., Silva, I., Morales, D., Lavanderos, B., Álvarez, A., Saldías, M. P., Pulgar, E., Cruz, P., Maureira, D., Flores, G., Colombo, A., Blanco, C., Contreras, H. R., Jaña, F., Gallegos, I., Concha, M. L., Vergara-Jaque, A., Poblete, H., González, W., Varela, D., Trimmer, J. S., Cáceres, M., and Cerda, O. (2020).
FASEB J 34:7847-7865. PMID: 32301552.

Kv2.1 Channels Play Opposing Roles in Regulating Membrane Potential, Ca2+ Channel Function, and Myogenic Tone in Arterial Smooth Muscle.
O’Dwyer, S. C., Palacio, S., Matsumoto, C. T., Guarina, L., Klug, N., Tajada, S., Rosati, B., McKinnon, D., Trimmer, J. S., and Santana, L. F. (2020).
Proc. Nat. Acad. Sci. USA 117:3858-3866. PMID: 32015129.

Neuronal ER-PM Junctions Organized by Kv2-VAP Pairing Recruit Nir Proteins and Affect Phosphoinositide Homeostasis.
Kirmiz, M., Gillies, T. E., Dickson, E. J., and Trimmer, J. S. (2019).
J. Biol. Chem. 294:17735-17757. PMID: 31594866.

Kv2.1 Mediates Spatial and Functional Coupling of L-type Calcium Channels and Ryanodine Receptors in Neurons.
Vierra, N. C., Kirmiz, M., van der List, D., Santana, L. F., and Trimmer, J. S. (2019).
eLife 8:e49953. PMID: 31663850.

Phosphorylation of the HCN Channel Auxiliary Subunit TRIP8b is Altered in an Animal Model of Temporal Lobe Epilepsy and Modulates Channel Function.
Foote, K. M., Lyman, K. A., Han Y., Michailidis, I. E., Heuermann, R. J., Mandikian, D., Trimmer, J. S., Swanson, G. T., and Chetkovich D. M. (2019).
J. Biol. Chem. 294:15743-15758. PMID: 31492750

A Stochastic Model of Ion Channel Cluster Formation in the Plasma Membrane.
Sato, D., Hernández-Hernández, G., Matsumoto, C., Tajada, S., Moreno, C. M., Dixon, R. E., O’Dwyer, S., Navedo, M. F., Trimmer, J. S., Clancy, C. E., Binder, M. D., and Santana, L. F. (2019).
J. Gen. Physiol. 151:1116-1134. PMID: 31371391

Deep Sequencing of Somatosensory Neurons Reveals Molecular Determinants of Intrinsic Physiological Properties.
Zheng, Y., Liu, P., Bai, L., Trimmer, J. S., Bean, B. P., and Ginty, D. D. (2019).
Neuron 103:598-616. PMID: 31248728

Remodeling Neuronal ER-PM Junctions is a Conserved Nonconducting Function of Kv2 Plasma Membrane Ion Channels.
Kirmiz, M., Palacio, S., Thapa, P., King, A. N., Sack, J. T. and Trimmer, J. S. (2018).
Mol. Biol. Cell 29:2410-2432. PMID: 30091655

Identification of VAPA and VAPB as Kv2 Channel-Interacting Proteins Defining Endoplasmic Reticulum-Plasma Membrane Junctions in Mammalian Brain Neurons.
Kirmiz, M., Vierra, N. C., Palacio, S., and Trimmer, J. S. (2018).
J. Neurosci. 38:7562-7584. PMID: 30012696

Kv2 Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons.
Bishop, H.I., Cobb, M. M., Kirmiz, M., Parajuli, L., Mandikian, D., Philp, A. M., Melnik, M., Kuja-Panula, J., Rauvala, H., Shigemoto, R., Murray, K. D., and Trimmer, J. S. (2018).
Front. Mol. Neurosci. 11:1. DOI: 10.3389/fnmol.2018.00001. PMID: 29403353.

Heterogeneity in Kv2 Channel Expression Shapes Action Potential Characteristics and Firing Patterns in CA1 Versus CA2 Hippocampal Pyramidal Neurons.
Palacio, S., Chevaleyre, V., Brann, D. H., Murray, K. D., Piskorowski, R. A., and Trimmer, J. S. (2017).
eNeuro 4(4). pii: ENEURO.0267-17.2017. PMID: 28856240.

Cell Cycle-Dependent Changes in Localization and Phosphorylation of the Plasma Membrane Kv2.1 K+ Channel Impact Endoplasmic Reticulum Membrane Contact Sites in COS-1 Cells.
Cobb, M. M., Austin, D. C., Sack, J. T., and Trimmer, J. S. (2015).
J. Biol. Chem. 290:29189-29201. PMID: 26442584.

Distinct Cell- and Layer-Specific Expression Patterns and Independent Regulation of Kv2 Channel Subtypes in Cortical Pyramidal Neurons.
Bishop, H. I., Guan, D., Bocksteins, E., Parajuli, L., Murray, K. D., Cobb, M. M., Misonou, H., Zito, K., Foehring, R. C., and Trimmer, J. S. (2015).
J. Neurosci. 35:14922-14942. PMID: 26538660. Cover article.

A Novel Epileptic Encephalopathy Mutation in KCNB1 Disrupts Kv2.1 Ion Selectivity, Expression, and Localization.
Thiffault, I., Speca, D. J., Austin, D. C., Cobb, M. M., Eum, K. S., Safina, N. P., Grote, L., Farrow, E. G., Miller, N., Soden, S., Kingsmore, S. F., Trimmer, J. S., Saunders, C. J., and Sack J.T. (2015).
J. Gen. Physiol. 146: 399-410. PMID: 26503721.

Tool development: renewable affinity reagents

Developing a Toolbox of Antibodies Validated for Array Tomography-Based Imaging of Brain Synapses.
Micheva, K. D., Gong, B., Collman, F, Weinberg, R. J., Smith, S. J., Trimmer, J. S., and Murray, K. D. (2023).

High‑Volume Hybridoma Sequencing on the NeuroMabSeq Platform Enables Efficient Generation of Recombinant Monoclonal Antibodies and ScFvs for Neuroscience Research.
Mitchell, K. G., Gong, B., Hunter, S. S., Burkart-Waco, D., Gavira-O’Neill, C. E., Bzymek, M., Goethel, M. E., Templeton, K. M., MacNiven, L. M., Murray, K. D., Settles, M. L., Froenicke, L., and Trimmer, J. S. (2023).
Sci. Rep.
13:16200. PMID: 37758930.

Genetically Encoded Intrabodies as High-Precision Tools to Visualize and Manipulate Neuronal Function. 
Trimmer, J. S. (2022).
Sem. Cell Dev. Biol.
126:117-124. PMID: 34782184.

Controlling Ion Channel Function with Renewable Recombinant Antibodies.
Colecraft, H. M., and Trimmer, J. S. (2022).
J. Physiol.
600:2023-2036. PMID: 35238051.

Development, Screening, and Validation of Camelid-derived Nanobodies for Neuroscience Research.
Gavira-O’Neill, C. E., Dong, J.-X., and Trimmer, J. S. (2020).
Curr. Prot. Neurosci.
94: e107.doi: 10.1002/cpns.107. PMID: 33185319.

Recombinant Antibodies in Basic Neuroscience Research.
Trimmer, J. S. (2020).
Curr. Prot. Neurosci.
94:e106.doi: 10.1002/cpns.106. PMID: 33151027.

Perspective: The Antibody Validation Webinar Series from The Antibody Society.
Voskuil, J. L. A., Bandrowski, A., Begley, C. G., Bradbury, A. R. M., Chalmers, A. D., Gomes, A. V., Hardcastle, T., Lund-Johansen, F., Plükthun, A., Roncador, G., Solache, A., Taussig, M. J., Trimmer, J. S., Williams, C., and Goodman, S. L. (2020).
12:1794421. PMID: 32748696.

BRAIN Initiative: Cutting-edge Tools and Resources for the Community.
Litvina, E., Adams, A., Barth, A., Bruchez, M., James, C., Dupre, K., Frank, L., Gates, K., Harris, K., Joo, H., Lichtman, J., Ramos, K., Sejnowski, Trimmer, J. S., White, S., and Koroshetz, W. (2019).
J. Neurosci.
39:8275-8284. PMID: 31619497.

A Toolbox of Nanobodies Developed and Validated for Diverse Neuroscience Research Applications.
Dong, J.-X., Lee, Y., Kirmiz, M., Palacio, S., Dumitras, C., Moreno, C. M., Sando, R., Santana, L. F., Südhof, T. C., Gong, B., Murray, K. D., and Trimmer, J. S. (2019).
8:e48750. PMID: 31566565.

A Toolbox of IgG Subclass-Switched Recombinant Monoclonal Antibodies for Enhanced Multiplex Immunolabeling of Brain.
Andrews, N. P., Boeckman, J. X., Manning, C. F., Nguyen, J. T., Bechtold, H., Dumitras, C., Gong, B., Nguyen, K., van der List, D., Murray, K. D., Engebrecht, J. and Trimmer, J. S. (2019).
8:e43322. PMID: 30667360.

Antibody Validation: A View from the Mountains. [Review]
Taussig, M. J., Fonseca, C., and Trimmer, J.S. (2018).
New Biotech.
45:1-8. PMID: 30086383.

Developing High-Quality Mouse Monoclonal Antibodies for Neuroscience Research-Approaches, Perspectives and Opportunities. [Review]
Gong, B., Murray, K. D., and Trimmer, J.S. (2016).
New Biotech. 33:551-564. PMID: 26644354.

Benefits and Pitfalls of Secondary Antibodies: Why Choosing The Right Secondary is of Primary Importance.
Manning, C. F., Bundros, A. M., and Trimmer, J.S. (2012)
PLoS ONE 7:e38313. PMID:22675541.

A searchable database of publications resulting from the use of monoclonal antibodies that we developed and that are available through the UC Davis/NIH NeuroMab Facility can be found here:

Latest publications

Publication: Neuronal ER-PM junctions organized by Kv2-VAP pairing recruit Nir proteins and affect phosphoinositide homeostasis

Abstract.  The association of plasma membrane (PM)-localized voltage-gated potassium (Kv2) channels with endoplasmic reticulum (ER)-localized vesicle-associated membrane protein-associated proteins VAPA and VAPB defines […]

Publication: Kv2.1 mediates spatial and functional coupling of L-type calcium channels and ryanodine receptors in neurons

Abstract. The voltage-gated K+ channel Kv2.1 serves a major structural role in the soma and proximal dendrites of mammalian brain neurons, tethering the […]

Publication: A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in mammalian brain neurons

Abstract.  Nanobodies (nAbs) are small, minimal antibodies that have distinct attributes that make them uniquely suited for certain biomedical research, diagnostic and therapeutic […]

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