Research publication

Antibody validation: a view from the mountains

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Abstract: Validation of antibodies and other protein binders is a subject of pressing concern for the research community and one which is uppermost in the minds of all who use antibodies as research and diagnostic reagents. Assessing an antibody’s fitness for purpose includes accurate ascertainment of its target specificity and suitability for the envisaged task. […]

Research publication

Remodeling neuronal ER-PM junctions is a conserved nonconducting function of Kv2 plasma membrane ion channels

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Abstract: The endoplasmic reticulum (ER) and plasma membrane (PM) form junctions crucial to ion and lipid signaling and homeostasis. The Kv2.1 ion channel is localized at ER-PM junctions in brain neurons and is unique among PM proteins in its ability to remodel these specialized membrane contact sites. Here, we show that this function is conserved […]

Announcement

Trimmer lab to head BRAIN Initiative consortium to develop nanoscale immunolabeling reagents

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The Trimmer lab received a five year U24 award from the NIH National Institute of Neurological Disorders and Stroke for their BRAIN Initiative project titled “Recombinant Immunolabels for Nanoprecise Brain Mapping Across Scales.” This award is part of theBRAIN Initiative U24 grant program that provides support to labs that are producing resources that serve the […]

Research publication

Identification of VAPA and VAPB as Kv2 Channel-Interacting Proteins Defining Endoplasmic Reticulum-Plasma Membrane Junctions in Mammalian Brain Neurons

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SIGNIFICANCE STATEMENT Our study identified the endoplasmic reticulum (ER) proteins vesicle-associated membrane protein-associated proteins isoforms A and B (VAPA and VAPB) as proteins copurifying with the plasma membrane (PM) Kv2.1 ion channel. We found that expression of Kv2.1 recruits VAPs to ER-PM junctions, specialized membrane contact sites crucial to distinct aspects of cell function. We […]

Announcement

Trimmer lab receives an NIH R21 award to define the proteomic composition of neuronal ER-PM junctions

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The Trimmer lab received funding from the NIH National Institute of Neurological Disorders and Stroke for their project titled “Defining the Proteomic Composition of ER:Plasma Membrane Junctions in Brain Neurons.” This award is part of the R21 grant program that aims encourage exploratory/developmental research by providing support for the early and conceptual stages of project […]

Announcement

Santana and Trimmer labs receive R01 award from NIH to study calcium signaling in smooth muscle

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The Santana and Trimmer labs received a multi-PI R01 award from the NIH National Heart, Lung, and Blood Institute for their project titled “Tuning L-Type Ca Channel Activity in Arterial Smooth Muscle by Kv Channel-Mediated Clustering.” This award is part of the R01 grant program that provides support for health-related research. Abstract Dihydropyridine-sensitive, L-type Cav1.2 […]