Trimmer lab recombinant monoclonal antibody plasmids now available through Addgene
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The first set of plasmids encoding recombinant monoclonal antibodies has been made available through Addgene. https://www.addgene.org/James_Trimmer/
Michael Kirmiz awarded PhD
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Michael Kirmiz was awarded his PhD from the Biochemistry, Molecular, Cellular and Developmental Biology Graduate Group on November 27, 2018. Michael will be pursuing postdoctoral studies in Rich Lewis’ laboratory at Stanford School of Medicine starting January 2019.
Antibody validation: a view from the mountains
Posted onAbstract: Validation of antibodies and other protein binders is a subject of pressing concern for the research community and one which is uppermost in the minds of all who use antibodies as research and diagnostic reagents. Assessing an antibody’s fitness for purpose includes accurate ascertainment of its target specificity and suitability for the envisaged task. […]
Nick Vierra awarded NRSA postdoc fellowship
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Congratulations to Dr. Nicholas Vierra on receiving a Postdoctoral National Research Service Award from NIH. This award provides up to three years of funding to individual postdoctoral researchers “to broaden their scientific background and extend their potential for research in specified health-related areas.”
Remodeling neuronal ER-PM junctions is a conserved nonconducting function of Kv2 plasma membrane ion channels
Posted onAbstract: The endoplasmic reticulum (ER) and plasma membrane (PM) form junctions crucial to ion and lipid signaling and homeostasis. The Kv2.1 ion channel is localized at ER-PM junctions in brain neurons and is unique among PM proteins in its ability to remodel these specialized membrane contact sites. Here, we show that this function is conserved […]
Trimmer lab to head BRAIN Initiative consortium to develop nanoscale immunolabeling reagents
Posted onThe Trimmer lab received a five year U24 award from the NIH National Institute of Neurological Disorders and Stroke for their BRAIN Initiative project titled “Recombinant Immunolabels for Nanoprecise Brain Mapping Across Scales.” This award is part of theBRAIN Initiative U24 grant program that provides support to labs that are producing resources that serve the […]
Identification of VAPA and VAPB as Kv2 Channel-Interacting Proteins Defining Endoplasmic Reticulum-Plasma Membrane Junctions in Mammalian Brain Neurons
Posted onSIGNIFICANCE STATEMENT Our study identified the endoplasmic reticulum (ER) proteins vesicle-associated membrane protein-associated proteins isoforms A and B (VAPA and VAPB) as proteins copurifying with the plasma membrane (PM) Kv2.1 ion channel. We found that expression of Kv2.1 recruits VAPs to ER-PM junctions, specialized membrane contact sites crucial to distinct aspects of cell function. We […]
Trimmer lab receives an NIH R21 award to define the proteomic composition of neuronal ER-PM junctions
Posted onThe Trimmer lab received funding from the NIH National Institute of Neurological Disorders and Stroke for their project titled “Defining the Proteomic Composition of ER:Plasma Membrane Junctions in Brain Neurons.” This award is part of the R21 grant program that aims encourage exploratory/developmental research by providing support for the early and conceptual stages of project […]
Santana and Trimmer labs receive R01 award from NIH to study calcium signaling in smooth muscle
Posted onThe Santana and Trimmer labs received a multi-PI R01 award from the NIH National Heart, Lung, and Blood Institute for their project titled “Tuning L-Type Ca Channel Activity in Arterial Smooth Muscle by Kv Channel-Mediated Clustering.” This award is part of the R01 grant program that provides support for health-related research. Abstract Dihydropyridine-sensitive, L-type Cav1.2 […]